Regulatory

Part:BBa_K1825007:Design

Designed by: Jonathan Asmund Arnesen   Group: iGEM15_UNIK_Copenhagen   (2015-09-05)


ZmUbi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 902
    Illegal XhoI site found at 656
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 733
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence used in our experiments had a large intron region. This region has been removed due to the presence of illegal restriction sites in the intron.


Source

Zea mays, corn.

References